Metabolomic Response to Drought Stress in Belosynapsis ciliata (Blume) ‘Qiuhong’

The drought stress responses of plants are complex regulatory mechanisms that include various physiological reflected by the global metabolic status. Metabolomics is an effective, analytical, and instrumental technique for informatics/statistics acquisition comprehensive information on metabolites. We investigate effect a Belosynapsis ciliata cultivar, ‘Qiuhong’ (a drought-tolerant cultivar), using liquid chromatography-mass spectrometry based widely targeted metabolomic approach. leaves subjected to 15- 30-day treatments then compared control group without rehydration group. In total, 290 differentially accumulated metabolites were detected between normal conditions through multivariate statistical analyses, which 65 (36 upregulated 29 downregulated) highlighted their significant contribution tolerance, including anthocyanin (peonidin 3-O-galactoside) caused purple-red hue in under stress. addition, we found two significantly altered pathways (citrate cycle purine metabolism) related enhanced tolerance plants. Notably, synthesis three compounds (p-coumaroyl putrescine, apigenin 6-C-glucoside, β-nicotinamide mononucleotide) was specifically induced drought-treated ‘Qiuhong’, indicating critical roles resistance. Our results provide foundation further research drought-resistant B. ciliata.